Continuous Wheat/Soybean Cropping Influences Soybean Yield and Rhizosphere Microbial Community Structure and Function

Wheat/soybean rotation is an important double-cropping system in the Huang-Huai-Hai plain of China. Continuous soybean cropping could cause soil quality deterioration and plant growth inhibition. However, effects continuous wheat/soybean on rhizosphere microbes remain largely unknown. In this study, we compared yield microbial community between winter wheat/summer (W/S) with two harvests one year soybean-winter maize (W/S-W/M) four years. The results showed that W/S group significantly (p < 0.05) declined within first W/S-W/M higher fertility index than group. sequencing revealed had a impact fungal bacterial community. 22.08–23.01% alpha diversity community, but did not vary composition groups differed significantly. group, relative abundance growth-promoting fungi (e.g., Mortierella), nematophagous Plectosphaerella), biological control Coniothyrium) was observed. lignocellulose-degrading Trechispora, Myceliophthora, Botryotrichum, Coniochaeta) pathogenic Pyrenochaetopsis Cyphellophora) LEfSe analysis demonstrated Mortierella, Trechispora serve as crucial biomarkers. Mortierella positively associated available P levels negatively NO3−-N pH while opposite trend. findings study enhance current understanding mechanisms obstacles ensure sustainability agricultural production.